Measuring the Reference
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http://www.dvaction.org/dvaction/movies/reference/euc-NU-0064.mov

Place the reference solution in the sample holder. Note the position of the pen mark on the cuvette; the mark must face this direction each time the cuvette is placed in the holder.
Measure the reference solution. The signal obtained from this measurement will be subtracted from all sample measurements.
Remove the cuvette from the sample holder.